Beschreibung
Stop-flow fluorescence and photodiode array spectroscopy, with a millisecond time resolution, are used here to investigate refolding kinetics of the photoactive proton pump bacteriorhodopsin in mixed DMPC/CHAPS micelles from a partially denatured state in SDS. The study suggests that both the apoprotein folding and subsequent binding of retinal chromophore likely proceed via distinct multiple parallel pathways to generate the native helical bundle. Taken together, these data should have profound implications on the mechanisms of folding of proteins within biological membranes.
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Hersteller:
BoD - Books on Demand
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In de Tarpen 42
DE 22848 Norderstedt
Autorenportrait
Amjad Farooq is Associate Professor at the University of Miami Miller School of Medicine in Florida, USA. His laboratory applies a diverse array of biophysical techniques to study protein-ligand interactions at structural, energetic and kinetic level in the context of key cellular signaling circuitry pertinent to health and disease.
