Chloroplasts are essential for the survival and flourishing of life on Earth. Over the years, chloroplast biology has been studied in a variety of different organisms, leading to the significant disadvantage that findings which were made by using different experimental systems or species were not always directly cross-comparable.  The relatively recent adoption of Arabidopsis thaliana as the model organism of choice for plant science research, across the globe, has led to its emergence as a pre-eminent system for research on chloroplasts and other types of plastid.  In Chloroplast Research in Arabidopsis: Methods and Protocols, expert researchers bring together some of the most important, modern techniques and approaches for chloroplast research, with the unifying theme of Arabidopsis as the model system. Volume II explores topics such as multiprotein complexes, protein-protein interactions, omics and large-scale analyses, proteomics and suborganellar fractionation, as well as photosynthesis and biochemical analysis.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and convenient, Chloroplast Research in Arabidopsis: Methods and Protocols serves as an ideal reference for all researchers with a general interest in chloroplasts, plastids, or related processes.

Hersteller:
Humana Press in Springer Science + Business Media
juergen.hartmann@springer.com
Heidelberger Platz 3
DE 14197 Berlin

Part I: Multiprotein Complexes and Protein-Protein Interactions   1. One- and Two-Dimensional Blue Native (BN) PAGE and Immunodetection of Low-Abundance Chloroplast Membrane Protein Complexes             Shingo Kikuchi, Jocelyn Bedard, and Masato Nakai   2. Analysis of Thylakoid Protein Complexes by Two Dimensional Electrophoretic Systems             Sari Sirpiö, Marjaana Suorsa, and Eva-Mari Aro   3. Preparation of Multiprotein Complexes from Arabidopsis Chloroplasts Using Tandem Affinity Purification             Charles Andres, Birgit Agne, and Felix Kessler   4. Studying Interactions between Chloroplast Proteins in Intact Plant Cells Using Bimolecular Fluorescence Complementation (BiFC) and Förster Resonance Energy Transfer (FRET)             Jodi Maple and Simon G. Moller   5. Studying Chloroplast Protein Interactions In Vitro: An Overview of the Available Methods             Joanna Tripp and Enrico Schleiff   Part II: Omics and Large-Scale Analyses   6. Proteome Databases and Other Online Resources for Chloroplast Research in Arabidopsis             Diogo Ribeiro Demartini, Celia Regina Carlini, and Jay J. Thelen   7. Use of Transcriptomics to Analyse Chloroplast Processes in Arabidopsis             Tatjana Kleine and Dario Leister   8. Use of Non-Aqueous Fractionation and Metabolomics to Study Chloroplast Function in Arabidopsis             Peter Geigenberger, Axel Tiessen, and Jörg Meurer   9. Chloroplast Phenomics: Systematic Phenotypic Screening of Chloroplast Protein Mutants in Arabidopsis             Yan Lu, Linda J. Savage, and Robert L. Last   Part III: Proteomics and Suborganellar Fractionation   10. Preparation of Envelope Membrane Fractions from Arabidopsis Chloroplasts for Proteomic Analysis and Other Studies             Daniel Salvi, Lucas Moyet, Daphne Seigneurin-Berny, Myriam Ferro, Jacques Joyard, and Norbert Rolland   11. Preparation of Stroma, Thylakoid Membrane, and Lumen Fractions from Arabidopsis thaliana Chloroplasts for Proteomic Analysis             Michael Hall, Yogesh Mishra, and Wolfgang P. Schröder   12. Preparation of Plastoglobules from Arabidopsis Plastids for Proteomic Analysis and Other Studies             Celine Besagni, Lucia Eugeni Piller, and Claire Brehelin   13. Preparation and Proteomic Analysis of Chloroplast Ribosomes             Kenichi Yamaguchi   14. The Workflow for Quantitative Proteome Analysis of Chloroplast Development and Differentiation, Chloroplast Mutants, and Protein Interactions by Spectral Counting             Giulia Friso, Paul Dominic B. Olinares, and Klaas J. van Wijk   15. Use of Phosphoproteomics to Study Post-Translational Protein Modifications in Arabidopsis Chloroplasts             Anne Endler and Sacha Baginsky   Part IV: Photosynthesis and Biochemical Analysis   16. Use of a Pulse-Amplitude Modulated (PAM) Chlorophyll Fluorometer to Study the Efficiency of Photosynthesis in Arabidopsis Plants             Matthew D. Brooks and Krishna K. Niyogi   17. Gas Exchange Measurements for the Determination of Photosynthetic Efficiency in Arabidopsis Leaves             Giles Johnson and Erik Murchie   18. Measurement of the Î'pH and Electric Field Developed Across Arabidopsis Thylakoids in the Light             Steven M. Theg and Curtis Tom   19. Measurement of Chloroplast ATP Synthesis Activity in Arabidopsis             Aleel K. Grennan and Donald R. Ort   20. Methods for Analysis of Photosynthetic Pigments and Steady-State Levels of Intermediates of Tetrapyrrole Biosynthesis             Olaf Czarnecki, Enrico Peter, and Bernhard Grimm   21. Analysis of Starch Metabolism in Chloroplasts             Carmen Hostettler, Katharina Kölling, Diana Santelia, Sebastian Streb, Oliver Kötting, and Samuel C. Zeeman   22. The Analysis of Lipid Content a ...