Inhaltsangabe1. 3D Cell Culture: A Review of Current Approaches and Techniques John W. Haycock 2. Scaffolds for Tissue Engineering and 3D Cell Culture Eleonora Carletti, Antonella Motta, and Claudio Migliaresi 3. Tracking Nanoparticles in Three Dimensional Tissue Engineered Models Using Confocal Laser Scanning Microscopy Vanessa Hearnden, Sheila MacNeil, and Giuseppe Battaglia 4. Using Immuno-Scanning Electron Microscopy for the Observation of Cell Adhesion Interaction at the Nano- and Microscale in S-phase Cells Manus J.P. Biggs, R. Geoff Richards, and Matthew J. Dalby 5. 3D Sample Preparation for Orthopedic Tissue Engineering Bioreactors Sarah H. Cartmell, Sarah Rathbone, Gemma Jones, and L. Araida Hidalgo-Bastida 6. Quantification of mRNA Using Real-Time PCR and Western Blot Analysis of MAPK Events in Chondrocyte/Agarose Constructs David A. Lee, June Brand, Donald Salter, Oto-Ola Akanji, and Tina T. Chowdhury 7. Genetic Modification of Chondrocytes Using Viral Vectors Teresa Coughlan, Aileen Crawford, Paul Hatton, and Michael Barker 8. Stem Cell and Neuron Co-Cultures for the Study of Nerve Regeneration Paul J. Kingham, Cristina Mantovani, and Giorgio Terenghi 9. Production of Tissue Engineered Skin and Oral Mucosa for Clinical and Experimental Use Sheila MacNeil, Joanna Shepherd, and Louise Smith 10. ThreeDimensional Alignment of Schwann Cells Using Hydrolysable Microfiber Scaffolds: Strategies for Peripheral Nerve Repair Celia MurrayDunning, Sally L. McArthur, Tao Sun, Rob McKean, Anthony J. Ryan, and John W. Haycock 11. Encapsulation of Human Articular Chondrocytes into 3D Hydrogel: Phenotype and Genotype Characterization Rui C. Pereira, Claudio Gentili, Ranieri Cancedda, Helena S. Azevedo, and Rui L. Reis 12. MicroStructured Materials and Mechanical Cues in 3D Collagen Gels James B. Phillips and Robert Brown 13. Organotypic and 3D Reconstructed Cultures of the Human Bladder and Urinary Tract Claire L. Varley and Jennifer Southgate 14. Ex Vivo Organ Culture of Human Hair Follicles: A Model Epithelial - Neuroectodermal - Mesenchymal Interaction System Desmond J. Tobin 15. Human Endothelial and Osteoblast Co-Cultures on 3D Biomaterials Ronald E. Unger, Sven Halstenberg, Anne Sartoris, and C. James Kirkpatrick 16. Assessment of Nanomaterials Cytotoxicity and Internalization Noha M. Zaki and Nicola Tirelli 17. Practical Aspects of OCT Imaging in Tissue Engineering Stephen J. Matcher 18. Osteogenic Differentiation of Embryonic Stem Cells in 2D and 3D Culture Lee Buttery, Robert Bielby, Daniel Howard, and Kevin Shakesheff 19. 3D Structuring of Biocompatible and Biodegradable Polymers via Stereolithography Andrew A. Gill and Frederik Claeyssens 20. Alvetex®: Polystyrene Scaffold Technology for Routine Three Dimensional Cell Culture Stefan Przyborski

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1. 3D Cell Culture: A Review of Current Approaches and Techniques John W. Haycock 2. Scaffolds for Tissue Engineering and 3D Cell Culture Eleonora Carletti, Antonella Motta, and Claudio Migliaresi 3. Tracking Nanoparticles in Three Dimensional Tissue Engineered Models Using Confocal Laser Scanning Microscopy Vanessa Hearnden, Sheila MacNeil, and Giuseppe Battaglia 4. Using Immuno-Scanning Electron Microscopy for the Observation of Cell Adhesion Interaction at the Nano- and Microscale in S-phase Cells Manus J.P. Biggs, R. Geoff Richards, and Matthew J. Dalby 5. 3D Sample Preparation for Orthopedic Tissue Engineering Bioreactors Sarah H. Cartmell, Sarah Rathbone, Gemma Jones, and L. Araida Hidalgo-Bastida 6. Quantification of mRNA Using Real-Time PCR and Western Blot Analysis of MAPK Events in Chondrocyte/Agarose Constructs David A. Lee, June Brand, Donald Salter, Oto-Ola Akanji, and Tina T. Chowdhury 7. Genetic Modification of Chondrocytes Using Viral Vectors Teresa Coughlan, Aileen Crawford, Paul Hatton, and Michael Barker 8. Stem Cell and Neuron Co-Cultures for the Study of Nerve Regeneration Paul J. Kingham, Cristina Mantovani, and Giorgio Terenghi 9. Production of Tissue Engineered Skin and Oral Mucosa for Clinical and Experimental Use Sheila MacNeil, Joanna Shepherd, and Louise Smith 10. Three-Dimensional Alignment of Schwann Cells Using Hydrolysable Microfiber Scaffolds: Strategies for Peripheral Nerve Repair Celia Murray-Dunning, Sally L. McArthur, Tao Sun, Rob McKean, Anthony J. Ryan, and John W. Haycock 11. Encapsulation of Human Articular Chondrocytes into 3D Hydrogel: Phenotype and Genotype Characterization Rui C. Pereira, Claudio Gentili, Ranieri Cancedda, Helena S. Azevedo, and Rui L. Reis 12. Micro-Structured Materials and Mechanical Cues in 3D Collagen Gels James B. Phillips and Robert Brown 13. Organotypic and 3D Reconstructed Cultures of the Human Bladder and Urinary Tract Claire L. Varley and Jennifer Southgate 14. Ex Vivo Organ Culture of Human Hair Follicles: A Model Epithelial - Neuroectodermal - Mesenchymal Interaction System Desmond J. Tobin 15. Human Endothelial and Osteoblast Co-Cultures on 3D Biomaterials Ronald E. Unger, Sven Halstenberg, Anne Sartoris, and C. James Kirkpatrick 16. Assessment of Nanomaterials Cytotoxicity and Internalization Noha M. Zaki and Nicola Tirelli 17. Practical Aspects of OCT Imaging in Tissue Engineering Stephen J. Matcher 18. Osteogenic Differentiation of Embryonic Stem Cells in 2D and 3D Culture Lee Buttery, Robert Bielby, Daniel Howard, and Kevin Shakesheff 19. 3D Structuring of Biocompatible and Biodegradable Polymers via Stereolithography Andrew A. Gill and Frederik Claeyssens 20. AlvetexÂ: Polystyrene Scaffold Technology for Routine Three Dimensional Cell Culture Stefan Przyborski