The use of nucleic acid or gene probes as cloning or diagnostic tools has promoted significant advances in human genome mapping and in the isolation and manipulation of genes generally. In Gene Probes, Marilena Aquino de Muro and Ralph Rapley have brought together an outstanding collection of time-tested protocols for designing and using gene probes in a wide variety of applications. The applications covered range from searching for specific genes in the human chromosome to the detection of microorganisms and their toxins in the environment and in food samples, as well as in the diagnosis of human disease. Each cutting-edge method includes practical hints and tips and is described in step-by-step detail by an expert who not only routinely uses the technique, but has also optimized it for experimental success. Helpful tutorials explain the principles of gene probe design, labeling, detection, target formation, and hybridization conditions. Comprehensive and versatile, Gene Probes: Principles and Protocols provides a cutting-edge practical resource for all biochemists, microbiologists, and molecular biologists who are using this powerful new technology to advance medical, environmental, and food research today.

InhaltsangabeTarget Format and Hybridization Conditions Alex Reid End Labeling Procedures: An Overview Elena Hilario Photobiotin Labeling Elena Hilario Nick Translation Alex Reid Random Primed Labeling Alex Reid Design and Evaluation of 16S rRNA-Targeted Oligonucleotide Probes for Fluorescence In Situ Hybridization Philip Hugenholtz, Gene W. Tyson, and Linda L. Blackall Evaluation of Alterations in the Tumor Supressor Genes INK4A and INK4B in Human Bladder Tumors Irene Orlow and Carlos Cordon-Cardo The bTubulin Gene Region as a Molecular Marker to Distinguish Leishmania Parasites Alexis Mendoza-León, Luis Luis, and Clara Martínez Detection of Malignant Plasma Cells in the Bone Marrow and Peripheral Blood of Patients with Multiple Myeloma Ross D. Brown and P. Joy Ho Detection of Chlamydia trachomatis by DNA Amplification Peter Timms and Sarah Mathews Isolation and Identification of Campylobacter Species by Use of Selective Enrichment, Nucleic Acid Amplification Methods, and Gene Probes Alex van Belkum, Nicole van den Braak, Leen-Jan van Doorn, and Hubert Endtz Diagnosis of Human Papillomavirus Using In Situ Hybridization and In Situ Polymerase Chain Reaction Gerard J. Nuovo Quantitation of In Situ Hybridization Using Image Analysis of Radioactively Labeled RNA Probes Teresa Bisucci, Tim D. Hewitson, and Ian A. Darby Stable Isotope Probing for Detection of Methanotrophs After Enrichment with 13CH4 Stefan Radajewski and J. Colin Murrell Detection of Enterococci in Freshwater and Seawater (16S and 23S rRNA Enterococcus Oligonucleotide Probes) Gillian D. Lewis, Sally A. Anderson, and Susan J. Turner Analysis of 16S-23S rRNA Intergenic Spacer of Vibrio cholerae and Vibrio mimicus for Detection of These Species Jongsik Chun, Irma N. G.Rivera, and Rita R. Colwell Colony Hybridization of Bacterial Isolates with Burkholderia cepacia-Specific Probes Laura G. Leff In Situ Hybridization of Burkholderia cepacia Cells Christopher J. McNamara and Laura G. Leff Detection of Polychlorinated Biphenyl-Degrading Organisms in Soil John Sanseverino, Alice C. Layton, and Gary S. Sayler Detection of Single-Copy Genes in DNA from Transgenic Plants Lee C. Garratt, Matthew S. McCabe, J. Brian Power, and Michael R. Davey Differentiation Between Transcripts of Genes Belonging to Small Families During Fruit Ripening and Abscission Claudio Bonghi, Benedetto Ruperti, and Pietro Tonutti Polymerase Chain Reaction Detection of Invasive Shigella and Salmonella enterica in Food Keith A. Lampel and Palmer A. Orlandi Polymerase Chain Reaction for Detection of Listeria monocytogenes Debra K. Winters

Leseprobe

Target Format and Hybridization Conditions Alex Reid End Labeling Procedures: An Overview Elena Hilario Photobiotin Labeling Elena Hilario Nick Translation Alex Reid Random Primed Labeling Alex Reid Design and Evaluation of 16S rRNA-Targeted Oligonucleotide Probes for Fluorescence In Situ Hybridization Philip Hugenholtz, Gene W. Tyson, and Linda L. Blackall Evaluation of Alterations in the Tumor Supressor Genes INK4A and INK4B in Human Bladder Tumors Irene Orlow and Carlos Cordon-Cardo The b-Tubulin Gene Region as a Molecular Marker to Distinguish Leishmania Parasites Alexis Mendoza-Leon, Luis Luis, and Clara Martínez Detection of Malignant Plasma Cells in the Bone Marrow and Peripheral Blood of Patients with Multiple Myeloma Ross D. Brown and P. Joy Ho Detection of Chlamydia trachomatis by DNA Amplification Peter Timms and Sarah Mathews Isolation and Identification of Campylobacter Species by Use of Selective Enrichment, Nucleic Acid Amplification Methods, and Gene Probes Alex van Belkum, Nicole van den Braak, Leen-Jan van Doorn, and Hubert Endtz Diagnosis of Human Papillomavirus Using In Situ Hybridization and In Situ Polymerase Chain Reaction Gerard J. Nuovo Quantitation of In Situ Hybridization Using Image Analysis of Radioactively Labeled RNA Probes Teresa Bisucci, Tim D. Hewitson, and Ian A. Darby Stable Isotope Probing for Detection of Methanotrophs After Enrichment with 13CH4 Stefan Radajewski and J. Colin Murrell Detection of Enterococci in Freshwater and Seawater (16S and 23S rRNA Enterococcus Oligonucleotide Probes) Gillian D. Lewis, Sally A. Anderson, and Susan J. Turner Analysis of 16S-23S rRNA Intergenic Spacer of Vibrio cholerae and Vibrio mimicus for Detection of These Species Jongsik Chun, Irma N. G. Rivera, and Rita R. Colwell Colony Hybridization of Bacterial Isolates with Burkholderia cepacia-Specific Probes Laura G. Leff In Situ Hybridization of Burkholderia cepacia Cells Christopher J. McNamara and Laura G. Leff Detection of Polychlorinated Biphenyl-Degrading Organisms in Soil John Sanseverino, Alice C. Layton, and Gary S. Sayler Detection of Single-Copy Genes in DNA from Transgenic Plants Lee C. Garratt, Matthew S. McCabe, J. Brian Power, and Michael R. Davey Differentiation Between Transcripts of Genes Belonging to Small Families During Fruit Ripening and Abscission Claudio Bonghi, Benedetto Ruperti, and Pietro Tonutti Polymerase Chain Reaction Detection of Invasive Shigella and Salmonella enterica in Food Keith A. Lampel and Palmer A. Orlandi Polymerase Chain Reaction for Detection of Listeria monocytogenes Debra K. Winters